|Title||Two Anaplasma phagocytophilum strains in Ixodes scapularis ticks, Canada.|
|Publication Type||Journal Article|
|Year of Publication||2014|
|Authors||Krakowetz CN, Dibernardo A, Lindsay LR, Chilton NB|
|Journal||Emerg Infect Dis.|
|Keywords||16S rRNA gene, Anaplasma phagocytophilum, arachnid, bacteria, blacklegged ticks, Canada, gram-negative, human granulocytic anaplasmosis, Ixodes scapularis, PCR-RFLP, single nucleotide polymorphism, SNP, vectorborne|
We developed PCR-based assays to distinguish a human pathogenic strain of Anaplasma phagocytophilum, Ap-ha, from Ap-variant 1, a strain not associated with human infection. The assays were validated on A. phagocytophilum-infected black-legged ticks (Ixodes scapularis) collected in Canada. The relative prevalence of these 2 strains in I. scapularis ticks differed among geographic regions.