Analysis of an Ordered, Comprehensive STM Mutant Library in Infectious Borrelia burgdorferi: Insights into the Genes Required for Mouse Infectivity.

TitleAnalysis of an Ordered, Comprehensive STM Mutant Library in Infectious Borrelia burgdorferi: Insights into the Genes Required for Mouse Infectivity.
Publication TypeJournal Article
Year of Publication2012
AuthorsLin, T, Gao, L, Zhang, C, Odeh, E, Jacobs, MB, Coutte, L, Chaconas, G, Philipp, MT, Norris, SJ
JournalPLoS ONE
Volume7
Start Pagee47532
Issue10
Date Published10/2012
Keywordsbiology, borrelia, burgdorferi, geens
Abstract

Using the infectious, moderately transformable B. burgdorferi derivative 5A18NP1 and signature-tagged versions of the Himar1 transposon vector pGKT, we have constructed a defined transposon library for the efficient genome-wide investigation of genes required for wild-type pathogenesis, in vitro growth, physiology, morphology, and plasmid replication.

URLhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0047532
DOI10.1371/journal.pone.0047532