Construction and characterization of a Borrelia burgdorferi strain with conditional expression of the essential telomere resolvase, ResT.

TitleConstruction and characterization of a Borrelia burgdorferi strain with conditional expression of the essential telomere resolvase, ResT.
Publication TypeJournal Article
Year of Publication2014
AuthorsBandy NJ, Salman-Dilgimen A, Chaconas G
JournalJ Bacteriol.
Volume196
Start Page2396
Issue13
Date Published07/2014
Abstract

Borrelia species are unique in the bacterial world in possessing segmented genomes which sometimes contain over 20 genetic elements. Most elements are linear and contain covalently closed hairpin ends requiring a specialized process, telomere resolution, for their generation. Treatment of DNA from the ResT-depleted strain with ResT in vitro revealed the presence of replicated telomeres expected in replication intermediates.

URLhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4054159/
DOI10.1128/JB.01435-13