A Borrelia burgdorferi mini‐vls system that undergoes antigenic switching in mice: investigation of the role of plasmid topology and the long inverted repeat

TitleA Borrelia burgdorferi mini‐vls system that undergoes antigenic switching in mice: investigation of the role of plasmid topology and the long inverted repeat
Publication TypeJournal Article
Year of Publication2018
AuthorsCastellanos, M, Verhey, TB, George Chaconas
JournalMolecular Microbiology
Volume109
Start Page710
Issue5
Date Published09/2018
Abstract

Amplicon sequencing using PacBio long read technology and analysis of the data with our recently reported pipeline and VAST software showed that the system undergoes switching in mice in both linear and circular versions and that the presence of the hairpin does not seem to be crucial in the linear version, however it is required when the topology is circular.

URLhttps://onlinelibrary.wiley.com/doi/10.1111/mmi.14071